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KMID : 0368420020450030182
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Journal of Plant Biology
2002 Volume.45 No. 3 p.182 ~ p.187
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Phylogeny of the Genus Goodyera(Orchidaceae; Cranichideae) in Korea Based on Nuclear Ribosomal DNA ITS Region Sequences
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Shin, Kee-Sun
Shin, Yong Kook/Kim, Joo-Hwan/Tae, Kyoung-Hwan
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Abstract
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We sequenced the nuclear ribosomal internal transcribed spacer (ITS) regions to determined the phylogenetic relationships of the several Goodyera species in Korea and to measure the extent of their differentiation region. ITS 1 was 238 to 239 bp long while ITS 2 was 258 to 259 bp. The 5.8S coding region was 156 bp long. Sequence divergences among species, calculated by Kimura´s two- parameter method, ranged from 0.0 to 5.4%. The most parsimonious tree, with a consistency index of 0.935 and a retention index of 0.937, was produced with 337 steps. Our ITS sequence results demonstrate the monophyly of Korean Goodyera and support previous morphological, geographical, and RAPD data analyses.
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KEYWORD
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